作者：刘小慧1，贺曼曼2，张伟1，冯运章1，李静1

单位：1.河北省邯郸市中心医院 普外二科，河北 邯郸056001； 2.河北省邯郸市中心医院 麻醉科，河北 邯郸 056001

Authors:  LIU Xiaohui1, HE Manman2, ZHANG Wei1, FENG Yunzhang1, LI Jing1

Unit:  1.Department 2 of General Surgery, Handan Central Hospital, Handan 056001, Hebei, China； 2.Department of Anesthesiology, Handan Central Hospital, Handan 056001, Hebei, China

摘要：

目的  检测基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)与上皮型钙黏蛋白(E-cadherin)在胃癌及转移淋巴结中的表达,探讨两者表达的临床意义。方法  选取2017年12月～2018年6月在邯郸市中心医院手术的56例胃癌患者,分别采集各56例胃癌组织、癌旁组织、正常胃黏膜组织及34例转移淋巴结,采用RT-PCR、免疫组织化学染色法分别检测上述组织中SDF-1与E-cadherin m RNA及蛋白的表达情况,分析两者表达与胃癌临床病理参数的关系。结果  SDF-1mRNA及蛋白在正常胃粘膜、癌旁组织、胃癌组织、转移淋巴结中的表达呈递增趋势,组间差异均有统计学意义(均P<0.05)。E-cadherin mRNA和蛋白在正常胃粘膜、癌旁组织、胃癌组织中的表达呈递减趋势,组间差异均有统计学意义(均P<0.05)。E-cadherin mRNA和蛋白在转移淋巴结中的表达高于胃癌组织,差异有统计学意义(均P<0.05)。胃癌组织中SDF-1、E-cadherin mRNA和蛋白的表达水平与肿瘤分化程度、浸润深度、TNM分期、淋巴结转移均密切相关(均P<0.05)。在mRNA和蛋白水平,胃癌组织中SDF-1与E-cadherin的表达均呈负相关(r=-0.441、r=-0.833,P<0.01); 转移淋巴结中SDF-1与E-cadherin的表达均呈正相关(r=0.617、r=0.451,P<0.01)。结论 SDF-1高表达和Ecadherin表达下调或缺失协同参与了胃癌的发生发展、侵袭转移过程。SDF-1可能通过上调Ecadherin的表达促进胃癌淋巴结转移灶形成。

关键词： 基质细胞衍生因子-1; 上皮型钙黏蛋白; 胃癌; 转移淋巴结

Abstract：

Objective  To detect the expression of stromal cell-derived factor-1 (SDF-1) and Ecadherin in gastric cancer and metastatic lymph nodes,and then explore the clinical significance of their expression. Methods  56 cases of gastric cancer undergoing operation in Handan Central Hospital from december 2017 to june 2018 were collected, including 56 specimens of gastric cancer, gastric mucosa adjacent to cancer, gastric normal mucosa and 34 specimens of metastatic lymph nodes. RT-PCR and Immunohistochemistry were performed in these tissues for the mRNA and protien expression of SDF-1 and E-cadherin. Results  The mRNA and protein expression of SDF-1 obviously increased in gastric normal mucosa, gastric mucosa adjacent to cancer, gastric cancer and metastatic lymph nodes by turns, the difference among these groups was significant (all P<0.05). The mRNA and protein expression of E-cadherin obviously decreased in gastric normal mucosa, gastric mucosa adjacent to cancer and gastric cancer by turns, the difference among these groups was significant (all P<0.05). The mRNA and protein expression of E-cadherin in metastatic lymph nodes were higher than that in gastric carcinoma (all P<0.05). The expression of SDF-1 and E-cadherin in gastric cancer on mRNA and protein levels were significantly correlated with tumor differentiation, depth of invasion, TNM stages and lymphatic metatasis (all P<0.05). The expression of SDF-1 and E-cadherin was negatively correlated in gastric cancer tissues on mRNA and protein levels (r=-0.441、-0.833, P< 0.05). The expression of SDF-1 and E-cadherin was positively correlated in metastatic lymph nodes tissues on mRNA and protein levels(r=0.617、0.451, P<0.01). Conclusions  The high expression of SDF-1 and the down-regulation or deletion of E-cadherin expression are involved in the occurrence, development, invasion and metastasis of gastric cancer. SDF-1 may promote the formation of lymph node metastasis in gastric cancer by up-regulating the expression of E-cadherin.

Key Words:  SDF-1; E-cadherin; Gastric cancer; Metastatic lymph nodes