作者：罗喜顺，王海鹏，朱袭嘉，李盛国，龙祥恺，蒋世宇，张琪琦

单位：桂林医学院第二附属医院 胃肠外科，广西 桂林 54199

Authors:  Luo Xishun, Wang Haipeng, Zhu Xijia, Li Shengguo, Long Xiangkai, Jiang Shiyu, Zhang Qiqi

Unit:  Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Guilin Medical University, Guilin Guangxi 54199, China

摘要：

目的  探究结直肠癌KRAS基因突变阳性与P53基因及MCC基因的相关性研究。方法  回顾性研究2017年3月至2020年7月于桂林医学院第二附属医院确诊为结直肠癌患者89例,将其中26例KRAS突变阳性患者纳入突变组,63例KRAS突变阴性且无其他突变患者纳入未突变组。采用荧光定量PCR Taqman-ARMS探针法测定KRAS基因突变;免疫组织化学方法对P53基因、MCC基因表达进行检测,比较组间的差异性与相关性。结果  通过对89例结直肠癌患者KRAS突变阳性率的分析,结果显示26(29.21%)KRAS突变阳性,未突变患者为63(70.79%);结直肠癌KRAS阳性率为29.21%;KRAS突变组MCC突变阳性效率6 (23.08%)显著低于未突变组27(42.86%)差异有统计学意义(P<0.05);KRAS突变组P53突变阳性效率8(30.77%)著低于未突变组40(63.49%)差异有统计学意义(P<0.05);KRAS突变与P53、MCC呈负相关。结论  结直肠癌KRAS突变与P53、MCC呈显著的负相关关系。

关键词：  结直肠癌; KRAS基因; TP53基因; MCC基因

Abstract：

Objective To explore the relationship between KRAS gene mutation and P53 gene and MCC gene in colorectal cancer. Methods  From March 2017 to July 2020, 89 patients with colorectal cancer in our hospital were retrospectively studied. Among them, 26 patients with positive KRAS mutation were included in the mutation group, and 63 patients with negative KRAS mutation and no other mutation were included in the non mutation group. The mutation of KRAS gene was detected by TaqMan arms probe, and the expression of P53 gene and MCC gene was detected by immunohistochemistry. Results  By analyzing the positive rate of KRAS mutation in 89 patients with colorectal cancer, the results showed that 26 (29.21%)patients with KRAS mutation were positive, 63 (70.79%)patients without KRAS mutation were positive, and the positive rate of KRAS mutation in colorectal cancer was 29.21%. The positive rate of MCC mutation in KRAS mutation group 6 (23.08%)was significantly lower than that in non mutation group 27 (42.86%), the difference was statistically significant (P<0.05); the positive rate of P53 mutation in KRAS mutation group 8 (30.77%)was significantly lower than that in non mutation group 40 (63.49%), the difference was statistically significant (P<0.05); there was a significant negative correlation between KRAS mutation and P53, MCC. Conclusion  KRAS mutation is negatively correlated with P53 and MCC in colorectal cancer.

Key Words:  Colorectal cancer; KRAS gene; TP53 gene; MCC gene