作者：吕佳乐1，王雅1，朱云娜2，梁路1，李宁3

单位：1.山西医科大学，山西 太原 030001；2.北京航天总医院病理科，北京 100076；3.山西医科大学第一医院 病理科，山西 太原 030001

Authors:  Lyu Jiale1, Wang Ya1, Zhu Yunna2, Liang Lu1, Li Ning3

Unit:  1.Shanxi Medical University, Taiyuan 030001, Shanxi, China；2.Department of Pathology, Beijing Aerospace General Hospital, Beijing 100076, China；3.Department of Pathology, the First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China

摘要：

目的 探讨胃腺癌组织中动力相关蛋白1(DRP1)、Ki-67表达情况及其临床意义。方法 采用免疫组织化学法检测DRP1在112例接受根治性手术切除的胃癌患者的胃腺癌组织及相应癌旁组织中的表达,检测Ki-67在胃腺癌组织中的表达。应用透射电镜观察胃腺癌组织超微结构的变化。结果 DRP1在胃腺癌组织中的阳性率明显高于癌旁组织(P<0.001); DRP1在浸润深度、TNM分期、淋巴结转移、脉管侵犯等情况不同的胃腺癌组织中表达有差异,且差异有统计学意义(P<0.05); Ki-67在年龄、分化程度、Lauren分型不同的胃腺癌组织中表达有差异,差异有统计学意义(P<0.05);DRP1表达情况与Ki-67呈正相关(r=0.474,P<0.001)。透射电镜下,胃腺癌细胞线粒体肿胀、膜缺失,线粒体嵴断裂。结论 DRP1在胃腺癌组织中高表达,且与较差的生物学行为相关。

关键词：动力相关蛋白1; Ki-67; 胃腺癌

Abstract：

Objective To investigate the expression of dynamin related protein 1(DRP1) and Ki-67 in gastric adenocarcinoma tissues and their clinical significance. Methods The expression of DRP1 and Ki-67 in 112 cases of gastric adenocarcinoma who underwent radical surgical resection were detected by immunohistochemistry. The ultrastructure of gastric adenocarcinoma was observed by transmission electron microscope. Results The positive rate of DRP1 in gastric adenocarcinoma tissues was significantly higher than that in adjacent tissues (P<0.001). DRP1 was expressed differently in gastric adenocarcinoma tissues with different infiltration depth, TNM stage, lymph node metastasis and vascular invasion, and the difference was statistically significant (P<0.05) ; Ki-67 was expressed differently in gastric adenocarcinoma tissues with different age, differentiation degree and Lauren type, and the difference was statistically significant (P<0.05) ; the expression of DRP1 was positively correlated with cell proliferation index Ki-67 (r=0.474, P<0.001) . Under transmission electron microscope, mitochondria of gastric adenocarcinoma cells were swollen, membrane-deficient and mitochondrial cristae broken. Conclusion DRP1 is highly expressed in gastric adenocarcinoma tissues and is associated with poor biological behavior.

Key Words:  Dynamin related protein 1; Ki-67; Gastric adenocarcinoma