作者：徐建波，彭建军，石鹏，欧信德，何裕隆

单位：中山大学附属第一医院 胃肠外科，广东 广州 510080

Authors:  XU Jianbo, PENG Jianjun, SHI Peng, OU Xinde, HE Yulong

Unit:  Gastrointestinal Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong, China; 

摘要：

目的 探讨Semaphorin-3B(SEMA3B)在胃癌细胞中的表达及其与胃癌患者临床病理特征及预后的关系。方法 用RT-qPCR检测20对新鲜的胃癌与癌旁组织中SEMA3B基因mRNA表达水平,用免疫组织化学的方法检测27对石蜡包埋的胃癌与癌旁组织样品中蛋白表达水平。同时利用TCGA和GEO数据库中的相关数据对胃癌与正常胃黏膜中SEMA3B基因mRNA水平进行差异分析。利用免疫组织化学的方法检测232例胃癌组织中SEMA3B蛋白的表达水平,进一步分析SEMA3B蛋白表达水平与胃癌患者的临床病理特征及预后的关系。结果 与正常胃黏膜相比,SEMA3B基因在胃癌组织中的mRNA和蛋白表达水平均显著降低。胃癌组织中,SEMA3B蛋白表达水平与肿瘤细胞分化程度(P<0.001)、肿瘤长径(P<0.05)、M分期(P<0.05)及TNM分期(P<0.05)显著相关。此外,SEMA3B在胃癌中的低表达与胃癌患者术后更高的死亡风险相关,多因素Cox回归分析表明SEMA3B蛋白表达水平可作为提示胃癌患者预后的独立因素(HR=1.616,95%CI:1.121-2.332,P=0.010)。结论 SEMA3B在胃癌组织中显著低表达,SEMA3B蛋白低表达与胃癌患者的不良预后密切相关。SEMA3B的表达水平可作为胃癌患者预后的分子指标。

关键词：  SEMA3B; 胃癌; 临床病理特征; 预后

Abstract：

Objective  To investigate the relationship between the expression of Semaphorin-3B (SEMA3B) and clinicopathological features and its effect on prognosis of patients with gastric cancer. Methods  Fresh-frozen tissue and Paraffin-embedded tissues samples were used to investigate SEMA3B mRNA and protein expression in gastric cancer tissues and adjacent normal tissues by reverse transcriptionquantitative real-time PCR (RT-qPCR)and immunohistochemical (IHC)staining, respectively. Datasets from The Cancer Genome Atlas (TCGA)and Gene Expression Omnibus (GEO)were also used to analyze SEMA3B mRNA expression in gastric cancer and normal gastric mucosa. The correlations between SEMA3B expression and clinicopathological characteristics and prognosis of patients with gastric cancer were investigated in 232 gastric cancer tissue samples by IHC staining. Results  The mRNA and protein expression of SEMA3B in gastric cancer were both significantly lower than normal gastric mucosa. In gastric cancer specimens, low SEMA3B expression was associated with poorly differentiated histological classification (P<0.001), larger tumor size (P<0.05) and advanced M stage (P<0.05) and TNM stage (P<0.05). Moreover, multivariate regression analysis indicated that low SEMA3B expression of gastric cancer was also associated with an increased risk of death (HR=1.616; 95%CI: 1.121-2.332, P=0.010). Conclusions Our results show that low level of SEMA3B correlates with poor prognosis in patients with gastric cancer. SEMA3B expression can serve as a prognostic biomarker for patients with gastric cancer.

Key Words:  SEMA3B; Gastric cancer; Clinicopathological features; Prognosis